Tth111 I (PflF I)

Origin:
E.coli strain that carries the cloned Tth111 I gene from Thermus Thermophilus 111

Concentration:
5000, u.a./ml

Recognition site:
GACN/NNGTC
CTGNN/NCAG

Reaction buffer:
GM-buffer Y, (33 mM Tris-acetatel (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol.

Storage temperature:
-20°C

Ligation:

After 2-fold overdigestion with enzyme 10% of DNA fragments can be ligated.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 65°C.

Optimum temperature:
65°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum: