Xma I

Origin:
Xanthomonas malvacearum  

Concentration:
10 u/µl

Recognition site:
C/CCGGG
GGGCC/C

Reaction buffer:
GM-buffer IV, (20 mM Tris-acetate【pH 7.9 @ 25°C】,50 mM potassium acetate, 10 mM magnesium acetate, 100 μg/ml BSA)

Storage conditions:
20 mM Tris-HCl (pH 7.5 @ 25°C), 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol.

Storage temperature:
-20°C

Ligation and recutting:
After 3-fold overdigestion with enzyme 95% of DNA fragments can be ligated. Of these 90% can be recut.

Non-specific hydrolysis:
More than 95% of DNA fragments (1 μg) digested with 10-fold excess enzyme can be ligated by T4 DNA ligase (400 U) at 16°C for 16 hr. Of the ligation products, more than 95% can be re-cut.

Optimum temperature:
37°C

Heat inactivation:
65°C for 20 min.

Enzyme activity in % of maximum: