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GeneMark 6X DNA Loading Dye is used for DNA electrophoresis in agarose gel. Add it directly to DNA sample in 1X final concentration, then load to the gel.

Storage: -20°C

150 mM EDTA, 30% Glycerol, 0.1% Bromophenol Blue, 0.1% Xylene cyanol F.F.

DL02-1, 1 ml

DL02-100, 100 ml

6X DNA SafeView Loading Dye

(2016停產)

The 6X DNA SafeView Loading Dye is for running DNA samples on an agarose gel. The 6X DNA SafeView Loading Dye contains three regular dyes and a safe, non-carcinogen green fluorescent dye. The three regular dyes are for monitoring the agarose gel while it is running, and the green fluorescent dye is to replace the toxic carcinogen ethidium bromide (EtBr). By using this special DNA loading dye, your agarose gel will reveal three different colors; purple, blue and yellow, while running. The gel can be directly visualized under LED or UV light without any extra staining steps. DNA stained with the fluorescent dye will emit a green light (530nm).

Storage: 4°C, avoid light.

GSL02-1, 1 ml

GSL02-5, 5 ml

GSL02-10, 10 ml

2 M Tris-acetate,

50 mM EDTA-Na2, pH 8.3±0.2

GB01-1, 1 L

TBE buffer is the electophoresisbuffer for polyacrylamidegels and agarosegels. TBE has a higher buffering capacity than TAE. Normally, it is applied in a concentration of 1X for polyacrylamide gels and 0.5X for agarose gels and "band shifts" (gel mobility shift assay).

0.45 M Tris,

0.1 M EDTA-Na2,

0.445 M Boric acid, pH 8.3

GB02-1, 1 L

3 M Sodium chloride,

0.3 M tri-Sodium citrate, pH 7.0

10X TE Buffer

(Tris-EDTA Buffer)

TE-buffer (pH 8.0) is the standard buffer solution for dissolving and storage of plasmid DNA or oligonucleotides, because nucleic acids are largely protected from degradation. TE buffer is prepared as a 10X concentrate and stored at room temperature.

0.1 M Tris,

EDTA-Na.H₂O, pH 8.0

Detection of specific sequences of DNA-fragments after gel electrophoresis.

3 M Sodium chloride,

0.2 M NaH₂PO₄.H₂O,

0.02 M EDTA-Na.H₂O,

pH 7.4

GB09-1, 1 L

TEN buffer is as lysisbuffer for eucaryoticcells, especially in the alkaline lysisprotocol for the M13 isolation. TEN buffer prepared as a 10X concentrate and stored at room temperature.

0.4 M Tris, 10 mM EDTA-Na.H₂O, 1.5 M Sodium Chloride, pH 7.5

Denhardt's solution is a blocking reagent for preventing nonspecific binding of nucleic acids to nitrocellulose or nylon membranes in hybridization experiments. Denatured DNA binds to nitrocellulose membranes, but not free RNA. Pretreatment of the membrane with Denhardt's solution prevents the binding of single stranded or (nonspecific) denatured DNA. The specific annealing of denatured DNA to its complementary DNA is not inhibited. The use of Denhardt's reagent is recommended for Northern hybridization, hybridization of RNA, "single copy Southern hybridization" or hybridization of DNA, immobilized on nylon membranes. Denhardt's solution is set up as a 100X stock solution.

Storage:  Filtered and stored at -20 ℃.

2% Ficoll 400, 2% polyvinylpyrolidone, 2% BSA fraction V in water

GM006-10, 10 ml

GM006-50, 50 ml

Nanogram quantities of DNA can be efficiently precipitated with the help of "carriers" (glycogen, yeast-tRNA). Glycogen is applied, when nucleic acids are precipitated with 0.5 M ammonium acetate and isopropanol. Since glycogen is not a nucleic acid, it does not interfere with enzyme reaction as yeast-tRNA may do. But it may interfere the interaction of proteins and DNA.  The working concentration is approximately 50 μg/ml of glycogen.

Storage:  -20℃

Concentration: 20 mg/ml

GM14

(8 x 0.5 ml )

Salmon sperm DNA is added to the pre-hybridization solution to prevent unspecific binding of probes to the membrane in Southern and Northern-hybridization. Salmon sperm DNA is sonified (fragments of 500~1000 base-pair) and extracted with phenol chloroform. The lyophilised DNA is dissolved in sterile bidistilled water. This DNA solution is used in a final concentration of 100 μg/ml. 

Storage:  -20℃

Concentration: 10 mg/ml