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  RNA Related Solutions
   

RNA Related Solutions

Product Name

Description

Ordering Info

Datasheet

RNase DNase Away Solution

Ensure that your PCR workstations, countertops and apparatus are free from DNA or RNase contamination by using RNase DNase Away Solution. This specially formulated surface decontaminant is designed to eliminate all DNA or RNase from glassware without having a residual effect on subsequent DNA samples. In fact, RNase DNase Away Solution is proving to be more effective at degrading DNA than autoclaving! Simply apply DNA & RNase away to the surfaces or labware to be decontaminated and rinse clear.

Storage: RT

GD0339-100            , 100 ml 

 

GD0339-500            , 500 ml

 

RNase A stock (DNase-free)

Ribonuclease A (RNaseA)  is an endoribonuclease, that specifically cleaves single-stranded RNA 3' to pyrimidine residues (cytosine, uracil). Thereby, it generates pyrimidine-3'-phosphate or oligonucleotides with terminal pyrimidine-3'-phosphates. The pH-optimum is in the range of 7.0-7.5. RNase A is used for the purification of RNase-free DNA, for the removal of non-hybridized regions of  RNA: DNA-hybrides or as a molecular weight marker. RNase A stock is prepared at concentrations of 4 or 10 mg/ml. Storage:  -20°C

RA01:                       4 mg/ml, 4 ml

 

RA02:                    10 mg/ml, 4 ml

 

Ribonuclease Inhibitor (DNase-Free)

RNase inhibitor is purified from human placenta or porcine liver. These inhibitors form a 1:1 complex with RNase A, and inhibit RNase activity non-competitively. The inhibitor can easily be removed from the reaction system by phenol extraction, and does not inhibit the RNase H activity of Reverse Transcriptase. This product is free of exonuclease, endonuclease, RNase activities, and DTT should be present for activation of this protein.

Concentration: 40 U/μl

 

RB0478-2KU

, 2000 U

 

RNA-SafeGuard Reagent (20X)

RNA-SafeGuard is a mixture of non-toxic reagents for storage and decontamination of RNase for purified RNA. The RNA stored in this reagent can be used in many enzymatic reactions, including cDNA synthesis, RT-PCR, in vitro transcription. RNA-SafeGuard can also be used for preparation of molecular buffer in stead of DEPC, or used for buffer incompatible with DEPC, or solution cannot be autoclaved.
Storage: -20°C

RG-1, 1 ml

RG-10, 10 ml

 

TriSolution Plus Reagent

TriSolution Plus Reagent is a mixture of phenol, guanidium thiocynate, buffers and stabilizers developed for the isolation of total RNA from tissue, cells, bacteria, plant, yeast and virus. The entire procedure for total RNA isolation using this single-step RNA undegraded and free of protein and DNA contamination. The RNA can subsequently be used for Northern analysis, dot hybridization, polyA+ selection, in vitro translation, RNase protection assays, molecular cloning and combined RT-PCR procedures without additional treatment with DNase.
Storage:   4°C

TS-100, 100 ml

TS-200, 200 ml

 

RNAfterTM

RNAfterTM  is a non-toxic reagent for storage of various animal or plant tissues, culture cells and bacteria for RNA purification without using liquid nitrogen or -70°Cfreezer. The sample can be stored in RNAfterTM reagent for a day at 37°C, 1 week at RT, one month at 4°C and indefinitely at -20℃. The purified RNA quality is as high as stored in liquid nitrogen.

Storage : RT

Note: This reagent is able to use in genomic DNA purification from various sample.

RA-100, 100 ml

RA-500, 500 ml

 

DEPC (0.1%) treated water 
DEPC may be used as an added precaution when  autoclaving may not be sufficient to eliminate sufficient RNase for some applications. DEPC-treated water is autoclaved pre- and post-packaging  to ensure sterility and inactivation of DEPC. 
DEPC-Treated Water is suitable for use in RNA related  experiments. 
 
Storage : 4℃ 
GB14, 1 L

Random Primer(N6) Random Primer are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer.

GM004, 25 μg

Oligo dT(18) The oligo dT primer consists 18 dT residues and is designed to polyA+ RNA for first-strand cDNA synthesis. Desalted and purified by gel purification. GM005, 25 μg

 
 
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