Agencourt SPRIstand 6 Position Tube Magnet - 6孔磁力架                    货号：A-A29182

Agencourt  AMPure XP Beads      我们给您推荐一款物美价廉，并被客户所称贊的产品即 EpiNext beads （A-P-1063）.

背景资料：The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.。

产品描述：Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NX^Pand FX^P for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods.The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

产品特点：

1.High recovery of amplicons, greater than 100 bp ；

2.Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants ；

3.Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days ；

4.Efficient recovery of double stranded and single stranded DNA templates ；

5.Consistent recovery throughout the kit’s 12 month shelf life ；

6.Faster manual and automated processing as compared to traditional post-PCR cleanup methods。

图片展示：

步骤流程	回收效果
1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate	Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
Agencourt SPRIstand 6 Position Tube Magnet - 6孔磁力架	6孔	PDF	A-A29182
Agencourt  AMPure XP Beads-PCR Purification-5 ml	139/278preps	PDF	A-A63880
Agencourt  AMPure XP Beads-PCR Purification-60 ml	1,666/3,333preps	PDF	A-A63881
Agencourt  AMPure XP Beads-PCR Purification-450 ml	12,500/25,000preps	PDF	A-A63882
基因组DNA提取试剂盒(磁珠法)	400 次	PDF	A-D3005
基因组DNA提取试剂盒(磁珠法)	100次	PDF	A-D3004
土壤基因组DNA快速提取试剂盒(离心柱型)	200次	PDF	A-D4003

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。