该试剂盒可用于在免疫沉淀实验流程中富集经 m6A 修饰的 RNA，可用于 NGS 或 RT-qPCR 等下游实验。在片段化的 RNA 样本中，经修饰的 RNA 可与 N6– 甲基腺苷抗体结合，从而被与抗体结合的 Protein G 磁珠富集。再经多轮洗涤与纯化步骤后，富集的 RNA 洗脱于无核酸酶水中，可用于进一步的分析。
产品描述：Each kit contains sufficient reagents to perform 10 x 2-round immunoprecipitations with starting amounts of up to 250 µg of ribosome depleted or poly A+ purified RNA. It contains sufficient reagents for 5 x 2-round immunoprecipitations when using up to 250 µg of total RNA.。
产品特点：
1·Complete protocol for enrichment of m6A-modified –RNA and analysis by RT- qPCR included；
2·RNA controls (m6A modified and unmodified RNA) enable monitoring of enrichment and depletion；
3.Antibody supplied in a ready-to-use solution form. 4.Optimized antibody and enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA；
5.Universal positive and negative controls are included, which are suitable for quantifying m6A from any species；
6.Strip-well microplate format makes the assay flexible for manual or high throughput analysis；
7.Simple, reliable, and consistent assay conditions；
8.Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis。
