m6A甲基化酶活性/抑制分析试剂盒(比色法) 货号：A-P-9019
Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)
 扫我收藏分享推荐，还有机会拿红包哦！
背景资料：N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes and also found in prokaryotes and virus. Recently, DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In human cells, the m6A modification is catalyzed by the methyltransferases (writers) such as METTL3/14, WTAP, RBM15/15B and KIAA1429, and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent demethylases (erasers) such as FTO, ALKBH5 and TET-like enzymes. It was shown that m6A methylases and demethylases play important roles in many biological processes, ranging from development and metabolism to fertility and participate in pathogenesis of multiple diseases including cancers and viral infections. The dynamic and reversible chemical m6A modification on DNA/RNA may also serve as a novel epigenetic marker of profound biological significance. Up-regulation of m6A modification was shown to increase virus replication and promote cancer growth. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls。
产品描述：This kit is designed for measuring total m6A methylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A methylases bind to and methylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。
产品特点：
1·Fast: Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours；
2·Robust: Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent；
3.Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro.
4.Sensitive and Specific: Novel assay principle allows to detect the m6A methylase-converted end product, which makes the assay much more specific than that for by-product measurement. The assay also allows high sensitivity to be achieved. The activity can be detected from as low as 2 µg of nuclear extracts or 50 ng of purified enzymes；
5.Quantitative: The assay standard is included, which allows the specific activity of m6A methylase to be quantified；
6.Flexible: Strip-well microplate format makes the assay flexible for manual or high throughput analysis。
数据分析：
| 标准曲线展示 |
标曲分析 |
 |
 |
| 使用m6A甲基化酶活性/抑制分析试剂盒(比色法) 针对重组的METTL3/14 完成了m6A 甲基化酶活性的检测.重组的人类METTL3/14酶在不同浓度下的数据. |
用m6A甲基化酶分析标准品生成标准曲线 |
参考文献：
1.Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| m6A甲基化酶活性/抑制分析试剂盒(比色法) |
96次 |
英文PDF-中文PDF |
A-P-9019-96 |
 |
| m6A甲基化酶活性/抑制分析试剂盒(比色法) |
48次 |
英文PDF-中文PDF |
A-P-9019-48 |
|
| m6A去甲基化酶活性/抑制分析试剂盒（比色法） |
96次 |
英文PDF-中文PDF |
A-P-9013-96 |
|
| m6A去甲基化酶活性/抑制分析试剂盒（比色法） |
48次 |
英文PDF-中文PDF |
A-P-9013-48 |
|
| m6A RNA甲基化定量检测试剂盒（荧光法） |
96次 |
英文PDF-中文PDF |
A-P-9008-96 |
|
| m6A RNA甲基化定量检测试剂盒（荧光法） |
48次 |
英文PDF-中文PDF |
A-P-9008-48 |
|
| 尿液样本 m6A RNA甲基化定量检测试剂盒（比色法） |
96次 |
英文PDF-中文PDF |
A-P-9015-96 |
|
| 尿液样本 m6A RNA甲基化定量检测试剂盒（比色法） |
48次 |
英文PDF-中文PDF |
A-P-9015-48 |
|
| 总体RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
| 总体RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
48次 |
PDF |
A-P-4059-48 |
|
| 总组蛋白H3K27乙酰化定量检测试剂盒（比色法） |
96次 |
PDF |
A-P-4059-96 |
|
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
12次 |
PDF |
A-P-1056A-12 |
| EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina） |
24次 |
PDF |
A-P-1056A-24 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
| 抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
| 抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
| 抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
| 抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
