SARS-CoV-2 IgG/IgM ELISA快速检测试剂盒 货号:A-D-1002
SeroFlash SARS-CoV-2 IgG/IgM ELISA Fast Kit
扫我收藏分享推荐,还有机会拿红包哦!
背景资料:In the process of pathogenic microorganism infection, IgG and IgM are the most commonly used antibody markers of infectious diseases. IgM, as the first antibody in the process of infection, is usually used as a marker of acute infection. With the development of infection, IgM concentration gradually decreases and disappears after the appearance of IgG. IgG usually exists in the body for a long time, even if the virus has been completely eliminated. Positive blood can be used as an indicator of infection and previous infection. Therefore, detecting SARS-CoV-2 IgG and IgM antibodies is of great clinical significance and could effectively control large-scale transmission of SARS-CoV-2。
产品描述:This kit uses an indirect ELISA technology to qualitatively measure the SARS-CoV-2 IgG and IgM in serum and plasma. The assay plate is coated with a recombinant SARS-CoV-2 antigen (Spike protein). In the assay, samples are added into the plate wells. An immuno-complex bound in the well will be formed by the coated-SARS-CoV-2 antigen and the SARS-CoV-2 IgG/IgM antibodies contained in the sample. Such immuno-complex will be recognized by the HRP-conjugated anti-human IgG or IgM antibody. The color will be generated by the interaction of the bound immune-complex with TMB substrate. The more SARS-CoV-2 IgG or IgM antibody there is, the stronger the blue coloration. The reaction is stopped with stop buffer, which turns the solution yellow. The signal intensity in the wells can then be read, spectrophotometrically using a microplate reader at a wavelength of 450 nm。
起始材料:This assay is suitable for human serum and plasma
产品特点:
1·Fast: The entire procedure can be completed in less than 45 min;
2·High Sensitivity: Detection limit (LoD) can be as low as 0.002 µg/ml SARS-CoV-2 IgG antibody;
3.High Specificity: Specific for SARS-CoV-2 IgG and IgM, with no cross-reactivity to many other viruses.
4.Precision: Inter-assay ≤ 8%; Intra assay: ≤ 12%;
5.Flexible: Strip-well microplate format makes the assay available for manual or high throughput analysis and allows for the detection of IgG or IgM alone or both IgG and IgM at the same time。
数据分析:
标准曲线展示 |
标曲分析 |
|
|
使用m6A甲基化酶活性/抑制分析试剂盒(比色法) 针对重组的METTL3/14 完成了m6A 甲基化酶活性的检测.重组的人类METTL3/14酶在不同浓度下的数据. |
用m6A甲基化酶分析标准品生成标准曲线 |
参考文献:
1.Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
SARS-CoV-2 IgG/IgM ELISA快速检测试剂盒 |
96次 |
英文PDF- |
A-D-1002-96 |
|
m6A甲基化酶活性/抑制分析试剂盒(比色法) |
96次 |
英文PDF-中文PDF |
A-P-9019-96 |
|
m6A甲基化酶活性/抑制分析试剂盒(比色法) |
48次 |
英文PDF-中文PDF |
A-P-9019-48 |
|
m6A去甲基化酶活性/抑制分析试剂盒(比色法) |
96次 |
英文PDF-中文PDF |
A-P-9013-96 |
|
m6A去甲基化酶活性/抑制分析试剂盒(比色法) |
48次 |
英文PDF-中文PDF |
A-P-9013-48 |
|
m6A RNA甲基化定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9008-96 |
|
m6A RNA甲基化定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9008-48 |
|
尿液样本 m6A RNA甲基化定量检测试剂盒(比色法) |
96次 |
英文PDF-中文PDF |
A-P-9015-96 |
|
尿液样本 m6A RNA甲基化定量检测试剂盒(比色法) |
48次 |
英文PDF-中文PDF |
A-P-9015-48 |
|
总体RNA甲基化极易定量检测试剂盒(荧光法) |
96次 |
英文PDF-中文PDF |
A-P-9009-96 |
|
总体RNA甲基化极易定量检测试剂盒(荧光法) |
48次 |
英文PDF-中文PDF |
A-P-9009-48 |
|
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) |
48次 |
PDF |
A-P-4059-48 |
|
总组蛋白H3K27乙酰化定量检测试剂盒(比色法) |
96次 |
PDF |
A-P-4059-96 |
|
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) |
12次 |
PDF |
A-P-1056A-12 |
EpiNext 高灵敏重亚硫酸盐测序试剂盒(Illumina) |
24次 |
PDF |
A-P-1056A-24 |
|
96孔DNA甲基化极速修饰试剂盒(磁珠法) |
96次 |
英文PDF-中文PDF |
A-P-1050-096 |
|
96孔DNA甲基化极速修饰试剂盒(磁珠法) |
192次 |
英文PDF-中文PDF |
A-P-1050-192 |
|
抗人C5a/C5a desArg单克隆抗体(克隆号2942) |
100ug |
|
C-HM2078 |
|
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记 |
100ug |
|
C-HM2022F |
|
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记 |
50ug |
|
C-HM2008 |
|
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记 |
50ug |
|
C-HM2006 |
|
抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下:
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |